Emma Stack, Medical Scientist, at work in the new Molecular Laboratory at UHL
THE capacity to test for Covid-19 at University Hospital Limerick has been boosted by the opening of a new state of the art Molecular Laboratory.
The facility, which cost around €4m to construct, also provides the potential, post-pandemic, to increase the scope of molecular testing in other infectious diseases.
Funded as part of the national pandemic response, development work on the laboratory commenced last autumn and since it opened Covid-19 testing capacity in the hospital has risen by 50% to between 400 and 450 tests per day.
And the new laboratory’s Whole Genome Sequencing capacity, with its ability to detect SARS-CoV-2 variants of concern, such as the Delta variant of the virus, and track outbreaks in a more accurate and timely manner, could position UHL as an important element in the national response to calls from the European Centre for Disease Control for tracking Covid-19 and its multiplicity of viral lineages.
Dr Patrick Stapleton (pictured below), Consultant Microbiologist, explained the rapid testing capacity for Covid-19 and the Whole Genome Sequencing capacity, are housed in a purpose-built facility that has been developed to the highest possible standards.
The 'gold-standard' PCR testing of nasal and throat swabs is considered one the most sensitive tests available for SARS-CoV-2. It includes four distinct phases prior to reporting as 'detected' or 'not detected'.
UL Hospitals say the laboratory team worked closely with HSE Capital and Estates to optimise this design of the state of the art Molecular Laboratory.
“For the most accurate results and to avoid any potential for cross-contamination, separate work areas in the laboratory were dedicated to each phase of the PCR testing process, with separate air pressures designed to extract any potential contaminants from other areas. Each area has dedicated lab coats and equipment, ” explained Colm McDonnell, Chief Medical Scientist.
UHL has one of Ireland’s busiest hospital Covid-testing services, Dr Stapleton added.
“Prior to the opening of the molecular lab, our serology and microbiology laboratories, supported by all lab disciplines, had already built that capacity from scratch, and we were delivering fast, accurate test results for people attending our hospitals, and for people in the local community. However, the new Molecular Lab enhances this with permanent facilities, designed to ensure that we have best possible practices, procedures and environments in place to prevent any contamination of samples,” she said.
The building, which stands on a site to the rear of the hospital campus, is connected to the hospital’s pneumatic chute system, which not only shortens the turnaround time of transporting samples to the laboratory, but also minimises footfall throughout the hospital building.
With all Covid-19 testing equipment moved to the Molecular Lab from its temporary home in the laboratory of the Clinical Education and Research Centre (CERC), UHL’s Covid-19 testing capacity is now under one roof.
The new facility is composed of the high throughput Abbott ‘Alinity M’ diagnostic instrument, a fully automated sample-to-result system; the Serosep RespiBio testing platform; and the GeneXpert and Luminex Aries testing platforms which provide random-access rapid test capacity as a complement to fixed daily runs of batch PCR tests.
With PCR test capacity now complemented by Whole Genome Sequencing, Dr Stapleton says that the new Molecular Laboratory has “huge potential” as a centre of molecular diagnostics, across all laboratory disciplines.
The WGS instrument in the UHL laboratory, the Oxford Nanopore MinION, operated by specialist scientist Dr Carolyn Meaney, is smaller than a child’s tablet.
With a capacity of 12-96 samples per week, it can not only efficiently detect the virus, but also traces its lineage, and provide even more detailed analysis, such as individual mutations within a virus strain, which can then be used by outbreak control teams to track the spread of outbreaks.
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